New Fly-TILL Service
Fred Hutchinson Cancer Research
Center
and
University of Washington Department
of Biology
Dear Drosophilists,
We are posting this to announce the opening of Fly-TILL, a new service for the Drosophila research
community. Fly-TILL is part of the larger Seattle TILLING Project
(STP), a joint facility of the Fred Hutchinson Cancer Research Center and the
University of Washington Department of Biology.
TILLING (Targeting Induced Local Lesions in Genomes) is a
high throughput method to identify EMS-induced mutations in genes of
interest. The strategy was
originally developed to identify mutations in Arabidopsis (for a description of
the methods see Till et al. 2003).
The methods have been adapted for use with Drosophila melanogaster at our facility using the Z3 collection, a set of
strains that carry heavily EMS-mutagenized Chromosome 3 (Koundakjian et al.
2003). As we announced at last
yearŐs Drosophila Meetings, we have performed a pilot screen of the Z3 lines
(Till et al. 2004). Based on this
screen, we expect Fly-TILL to be about as efficient as the Arabidopsis TILLING Project that has delivered over 5,000
mutations in over 400 genes so far.
To place your order, or learn more about Fly-TILL, go to http://tilling.fhcrc.org:9366/fly/
The Web site describes how TILLING works, what Fly-TILL expects to deliver, the characteristics of the
Zuker collection, answers to frequently asked questions and user policies.
Fly-TILL is a
service that must be entirely supported by user fees. The cost will be $2800 (U.S. dollars) to screen 3072 lines
or $5000 for the entire population of 6129. Orders will be filled on a
first-come, first-serve basis.
Sincerely,
The Fly-TILL team
References:
Till, B.J., Reynolds, S.H., Greene, E.A., Codomo, C.A.,
Enns, L.C., Johnson, J.E., Burtner, C., Odden, A.R., Young, K., Taylor, N.E.,
Henikoff, J.G., Comai, L., Henikoff, S. (2003) Large-scale discovery of induced point mutations with
high-throughput TILLING. Genome
Res. 13(3):524-30.
Till, B.J., Young, K., Bowers, E., Codomo, C.A., Burtner,
C., Hassinger, R., Greene, E.A., Comai, L., Zuker, C.S., Wakimoto, B.,
Henikoff, S. High-throughput
TILLING for functional genomics.
(2004) Abstract for the 45th
Annual Drosophila Research Conference.
Koundakjian, E.J., Cowan, D.M., Hardy, R.W., Becker, A.H.
(2004) The Zuker collection: a
resource for the analysis of autosomal gene function in Drosophila
melanogaster. Genetics 167(1):203-6.